It is used
to demonstrate Corynebacterium diphtheria.
Preparation
of Albert's stain:-
Albert's
stain- I
[ Toluidine
blue - 0.15 gm
Malachite
green-0.20 gm
Glacial
acetic acid - 1.0 ml
Alcohol(95%)-2.0
ml
Distilled water
- 100ml.]
Albert's stain-
II
Iodine-
2.0gm
Potassium iodide-
3.0gm
Distilled water
- 300 ml
Procedure:-
1. Taking
an inoculum from bacterial culture or sputum sample , a thin smear is made by
mixing a drop of normal saline on the
glass slide.
2. Heat fix
the smear by passing the slide over the flame gently.
3. Flood
the smear with Albert's stain solution-I for 5 minutes.
4. Wash
with water.
5. Flood
the smear with Albert's stain- II for 2 minutes.
6. Wash
with water, blot dry and Observe under microscope.
Appearance
of the organism:-
Metachromatic
granules- bluish black.
Body of the
organism - green or bluish green.
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